원문정보
초록
영어
Recently the in vitro reconstruction of anterior cruciate ligament (ACL) has been tried using tissue engineering, especially mesenchymal stem cell (MSC) is considered as a good cell source. Mesenchymal stem cells (MSCs) from bone marrow appear to be an attractive tool for use in tissue engineering and cell-based therapies due to their multipotent capacity. In this study we tried to find the effect of mechanical stimulus on the differentiation of bone marrow-derived MSC into ACL fibroblast-like cell using a cell training bioreactor imposing cyclic mechanical tension whose parameters were 12 cycles/min, 0.2 Hz, various strain. And MTT assay, FASC analysis, morphology analysis and RT-PCR were performed. it is thought that mechanical cyclic
tension induced the loss of stemness of MSC, therefore improved the differentiation into ACL fibroblast-like cell.