원문정보
초록
영어
Recently, researchers have focused on the regeneration of PDL using tissue engineering.
Ideally, barriers should facilitate cell attachment and migration of the progenitorcells from periodontium, since anchorage dependent cells need to adhere to substrate to be viable in promoting functions such as proliferation, migration, differentiation, and maturation. So, in this study we tried to make a composite scaffold composed of collagen nano-fiber on amniotic membrane (AM) using electrospinning for dental tissue engineering. Collagen solution was prepared (100 mg/ml of hexafluore-2-isopropalol) and then electrospun (15 kV, 5 ml/hr) onto lyophilized AM. After dehydrothermal treatment (vacuum oven at 105C for 24 hrs), nano-fiber scaffolds were crosslinked with 0.02% carbodiimide. And then they were washed with PBS and lyophilized for 48 h. And they were sterilized by γ- irradiation at 10 KGy. PDL was separated from the surface of the root and then digested in a solution of 3 mg/ml collagenase and 4 mg/ml protease (dispase) for 30 min at 37C. And then harvested tissue was placed onto culture dish
and kept in α-MEM/10%FBS containing antibiotics. PDL cells migrated out of from the
tissue and reached confluence at 14 days. PDL cells were seeded into nano-fiber scaffolds using Teflon ring at a density of 1105 cells/cm2 and cultured for 7 days. Cells at 3 to 4 passages were used for this experiment. We could make a composite scaffold whose breaking load was 1 ±0.3 N superior to nano-fiber scaffold without AM (0.1 ± 0.05 N). After seeding of PDL cell on each scaffold, they attached and proliferated well on both scaffolds. However nano-fiber
scaffold without AM was contracted after 5 days of culture, but composite nano-fiber scaffold was not contracted during all culture days. As a result, composite nano-fiber scaffold made by electrospinning collagen fiber on AM will be a suitable substrate for PDL cell culture finally resulting in a good Bio-PDL. This works was supported by a grant from the Korean Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (0405-BO01-0204-0006).