earticle

영어

Human bone marrow-derived mesenchymal stem cells (hMSCs) were differentiated into adipocytes, which were verified via the visualization of intracellular lipid vacuoles, by both Oil Red O staining and by RT-PCR mRNA (CD90, C/EBP, PPARγ, and lipoprotein lipase) detection. The protein profiles from the adipocytes differentiated from hMSC were determined by 2-D gel electrophoresis (2-DE), and the differentially expressed proteins were identified by MALDI-TOF and ESI-Q-TOF MS/MS analysis. Among these, the three up-regulated proteins (Calgizzarin, Kallikrein-9, and Ubiquitin) were newly identified from differentiated hMSC, which might prove to be useful biomarkers for the differentiation of hMSC into adipocytes. In addition, these proteins were confirmed to play important roles for adipogenic-differentiation, which will be able to help understanding of differentiation related in its extracellular matrix (ECM) not in signal transduction