원문정보
초록
영어
The purposeof this study was to evaluate the biocompatibility of silk and collagen in vitroby ACL and T lymphocytes cultures on scaffolds. And the angiogenesis effect of composite scaffolds was evaluated as an artificial ligament for an ACL reconstruction in vivo. Silk scaffold for the artificial ligament was knitted silk by hand and collagen coated. The composite silk scaffold was made with silk scaffold covered by a collagen-HA substrate. The initial attachment and roliferation of human ACL cells on the composite silk scaffold was superior to the silk scaffold. The immune response was higher on the both scaffolds after 72 h (p<0.05) compared with the control culture condition without scaffold by T lymphocyte cultures in vitro. But between silk and composite silk scaffold, there was no significant difference. The both artificial ligament, silk and composite silk scaffold were implanted in the removed ACL in knee of dogs and harvested 6 weeks later. As a gross appearance, an inflammatory tissue reaction such as synovitis was similar silk scaffold with silk composite scaffold. A histological evaluation of the artificial ligaments implants revealed the presence of monocytes in the silk composite scaffold, and an absence of giant cells in all cases. These results suggest that the collagen-HA substrate is biocompatible in vitro and enhance new blood vessel and collagen formation in vivo.