원문정보
초록
영어
The aim of this study was to evaluate the efficacy of our bioartificial liver (BAL) system, which contains aggregates of porcine hepatocytes, in a porcine model of fulminant hepatic failure (FHF).
Hepatocytes were isolated from 10kg pigs and cultured as spheroids through suspension culture using spinner flasks. The packed bed type BAL was a containing immobilized hepatocyte spheroids beads. The efficacy of the BAL was evaluated using 50kg pigs with ischemic liver failure by portocaval shunt and ligation of hepatic artery. The pigs were divided into three groups : control, Blank, BAL group. The survival of pigs with FHF was significantly prolonged by about 150% with BAL treatment as compared to blank group. In addition, intracranial pressure, blood ammonia, creatinine levels were lower in the BAL group than in control and
blank group, indicating metabolic activity of porcine hepatocytes in the bioreactor. As results of preclinical trial spheroid-based BAL system showed surpressed blood ammonia level and longer survival. This spheroid-based BAL system will be a good candidate for the treatment of fulminant hepatic failure patients.