원문정보
초록
영어
Umbilical cord blood(UCB) is a rich source of hematopoietic stem cells, with practical and ethical advantages1). Recently, it is known that non-hematopoietic stem cells are also included in UCB, which has been intensively investigated for therapeutic application2,3). We analyzed whether non-hematopoietic stem cells, which could be differentiated into various cell lineages are included in cryopreserved UCB. Seeded UCB-mononuclear cells formed adherent colonized cells in conditioned medium containing granulocyte macrophage colony stimulating factor. Over a 3- to 4-week culture period, colonized cells gradually developed into adherent fibroblast-like monolayer cells. After additional cultivation for several days to weeks in the differentiation medium containing hepatocyte growth factor, oncostatin M and fibroblast growth factor-44-6), the cells were confirmed to be differentiated into hepatocyte-like cells using Reverase
Transcriptase-Polymerase Chain Reaction, immunocytochemistry and enzyme linked immunosorbent assay. That is, hepatic-differentiated cells expressed hepatocyte-specific
markers and albumin secretion also occurred in these cells, suggesting that they are capable of differentiating into functional hepatic cells.