earticle

영어

Mesenchymal stem cells (MSCs) are a highly promising source of adult stem cells for purposes of cell therapy and tissue repair in the field of regenerative medicine1-3). Although the most studied and accessible source of MSC is the bone marrow, the clinical use of bone marrow-derived MSCs (BM-MSCs) has presented problems, including pain, morbidity, and the significant drop in cell number and proliferate/differentiation capacity with age4). We isolated, cultured, and characterized MSCs from a number of tissues;including umbilical cord5), cord blood6), and adipose tissues7) that were discarded routinely in the past, and evaluated the usefulness of these MSCs. Cord blood-derived MSCs (CB-MSCs), Cord Wharton's jelly-derived MSCs (WJ-MSCs), or adipose tissue-derived MSCs (ASCs) increased at an exponential rate up
to passage 7, passage 8, or passage 7, respectively. WJ-MSCs, CB-MSCs, and ASCs expressed CD73, CD90, and CD105 and were absent for CD14, CD31, and CD45. They are differentiated into osteoblast, adipocyte, and chondrogenic lineages under appropriate culture condition. In this study, like BM-MSCs, WJ-MSCs, CB-MSCs, and ASCs expressed similar cell surface antigens, were able to differentiate into mesenchymal lineages, and possessed highly proliferation potential. Therefore umbilical cord, cord blood, and adipose tissue may become useful alternative sources of MSCs to cell therapy and tissue repairin the field of regenerative medicine.