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논문검색

A cell-based assay system for high-throughput screening of anti-wrinkle agents in human dermal fibroblast transfectant cells

초록

영어

A cell-based assay system for monitoring COL1A2 promoter activity was developed to determine the influence of activated COL1A2 promoter in human dermal fibroblast cells. A pLuc-COL1A2 promoter plasmid that expresses the luciferase reporter gene in response to COL1A2 promoter activity was constructed. The pLuc-COL1A2 promoter plasmid and pCI-neo plasmid containing the neomycin phosphotransferase (NPT) gene for geneticin resistance in host cells were cotransfected into human dermal fibroblast cells. COL1A2 promoter activities were measured by luciferase reporter gene assay using a luminescence detection method. Fibroblast cell transfectants treated with TNF-α, known to be an inhibitor of COL1A2 promoter expression, showed a reduction of COL1A2 promoter activity in a concentration-dependent manner, whereas TGF-β, known to be a stimulator of COL1A2 promoter expression, increased COL1A2
activity in a concentration-dependent manner. This assay system could be used to quantitatively measure COL1A2 promoter activity in human dermal fibroblast cells and allow the screening of anti-wrinkle agents from various synthetic chemicals and natural products.

저자정보

  • Sungran Huh Biospectrum Life Science Institute
  • Jongsung Lee Biospectrum Life Science Institute
  • Eunsun Jung Biospectrum Life Science Institute
  • Yeonggeun Ham Biospectrum Life Science Institute
  • Sang Suk Kim Skincure Life Science Institute
  • Deokhoon Park Biospectrum Life Science Institute

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