원문정보
초록
영어
Exposure to UV appears to be the most important environmental factor involved in the development of skin photoaging. It has been known that oxidative stress induced by UV lead to the skin cell death, the formation of reactive oxygen species(ROS), and the increase of matrix metalloproteinases (MMPs). In our previous study, it has been shown that silkworm hemolymph
extract(SHEX) has anti-oxidative activities as determined by in vitro assay. In this study, we investigated the effect of SHEX on oxidative stress induced by UV in immortalized human keratinocytes, HaCaT. Treatment of HaCaT cells with SHEX inhibited UV-induced cell death and also inhibited the formation of ROS, which was determined using DCFH-DA. Moreover, there was a reduction in the expression of MMP-1(interstitial collagenase) induced by UV. To identify
the antioxidant compounds, SHEX was separated by gel permeable chromatography. Seven fractions were obtained, and the anti-oxidative activities of each fraction were assayed. These results suggest that SHEX contains some components protecting human skin cells against oxidative stress induced by UV and such components will be used as cosmetic supplements.