원문정보
초록
영어
Active compounds of coffee were prepared by supercritical fluid extraction. Various active compounds of coffee, such as fatty acids, α-tocopherol, and β -sitosterol were extracted by supercritical fluid at temperature of 50℃∼70℃ and pressure of 250 bar∼400 bar. The mechanism of melanogenesis by extracts were investigated. Inhibitory effects on melanogeneis were observed with in vitro cultures of B16 melanoma cells. Proteome analysis were made to explain
the inhibitory melanogenesis by supercritical fluid extracts. B16 melanoma cells treated with and without extracts were analyzed using 2-D PAGE. Isoelectric focusing was carried out using commercial Immobiline Dry Strips 3-10NL 13cm and SDS-PAGE was made using 18⨯24 cm gels1). Gels were stained with silver nitrate and analyzed by Image Master program2).