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Inhibitory effects and mechanism of aging by natural products were studied using proteome analysis. The fibroblast were cultured in Dulbecco's Modified Eagle's Medium(DMEM) with or without natural products. The proteome of cultured human fibroblast were profiled using 2D-PAGE. Isoelectric focusing was carried out using commercial immobiline dry strips 3-10NL 13cm.
SDS-PAGE was performed using 18 x 24cm, 10.5 % acrylamide gel1). Gels were stained with silver nitrate, analyzed by image master 2D software program. In conclusion, we could identify that many factors such as collagen, MMPs, which affect the skin aging, were up or down-regulated by natural products2).