earticle

영어

Neuronal SNARE proteins are crucial protein component in membrane fusion between presynaptic membrane and synaptic vesicle. The formation of SNARE assembly drives membrane fusion, leading to the formation of fusion pore through which neurotransmitters are released. SNARE complex is composed of 3 proteins(Snap25, VAMP, Syntaxin 1a) and generates fundamental force that is required for the membrane fusion. It is thought that the regulation of this SNARE assembly may inhibit membrane fusion leading to the inhibition of neurotransmitter release. In the present study, we have designed small peptide that regulates SNARE complex formation. High-throughput screening technology was also developed by mimicking SNARE-driven membrane fusion in vitro. Then, the developed fusion assay was applied to measure the efficacy of synthetic peptides on membrane fusion. Among various 17mer and 6mer peptides tested, we could draw 6mer synthetic peptide that harbors much stronger membrane fusion inhibition efficacy than the well-known "Argirelline." The found peptide might function as anti-wrinkle agent by blocking SNARE complex formation while Botox cleaves SNARE proteins. Further, the found peptide might be developed to potential pain-killer.