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Studies on the inhibitory effect of Alnus sp. extracts on tyrosinase and acetylcholinesterase activity

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In the present work, we report the results of a study aimed at evaluating the antioxidant activity, tyrosinase activity and the acetylcholinesterase inhibitory capacity of methanol, n-hexane, CH2Cl2, EtOAc and n-buOH extracts from Alnus sp. The EtOAc extract of Alnus sp. showed the highest radical scavenging activity (DPPH test) and superoxide anion radical scavenging activity values IC50 65.83 and 14.53 /ml, respectively. The inhibition of tyrosinase activity ㎍ was higher in the EtOAc extract. The highest activity was found for EtOAc with an IC50 130.05 ㎍/ml.
The plant of methanol, n-hexane, CH2Cl2, EtOAc and n-buOH extracts were tested for acetylcholinesterase inhibitory activity using the Ellman colorimetric method. The results showed that the MeOH extract, n-hexane extract and CH2Cl2 extracts from Alnus sp. at concentraction of 0.1 mg/ml inhibitied more than 80% of AChE activity. these finging may contribute to biological significance in that acetylcholinesterase (AChE) inhibitor has been used as a drug for the symptomatic treatment of Alzheimer's desease.

저자정보

  • Hye Jung CHOI Interdisciplinary Program in Biotechnology,Changwon National University
  • Dong Wan KIM Department of Microbiology, Changwon National University
  • Young Whan CHOI School of Bioresource Science, Busan National University
  • Jum Soon KANG School of Bioresource Science, Busan National University
  • Young-Guen LEE School of Applied Life Science, Busan National University
  • Woo Hong JOO Interdisciplinary Program in Biotechnology,Department of Microbiology, Changwon National University

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