earticle

영어

Silk Fibroin is made up of multiple repeats of the sequence of Ala–Gly–Ala–Gly–Ser-Gly-1),2). Thus, the compositions of the hydrolyzed peptides was found to be mixtures of short peptides varying by two residues of amino acid3). The hydrolyzed peptides of silk fibroin were hardly separated by conventional chromatography, because of structural similarity. This report describes the determination of the major components of fractionated silk peptides using isopropanol by Electrospray Ionization-Time Of Flight Mass Spectrometer(ESI-TOF MS). Silk peptide was solubilized in varying alcohol/water mixtures and precipitates were isolated. The yield of isolated peptide was determinated by dry weight and by UV-Vis Spectrometer. The
peptide compositions of the supernatant and the precipitate were identical in ethanol. However, the major composition of silk peptide of Gly, Ala, Ser, Tyr and Val residues was concentrated in the supernatant more than in the precipitate when using isopropanol. Thus, isopropanol solubilization can be used to enrich the composition of major peptide residues.