원문정보
초록
영어
The enzyme-catalyzed resolution of racemic compounds is one of methods for obtaining optically pure substances. Lipase is an enzyme that catalyzes the hydrolysis of (S)-Naproxen 2,2,2-trifluoroethyl thioester to (S)-Naproxen. Hydrolytic reaction of (R,S)-naproxen 2,2,2-trifluoroethyl thioester by lipase was carried out in water-saturated isooctane for 72 h. Rreaction conditions such as temperature, concentrations of substrate and enzyme, and agitation speed was optimized by using response surface methodology (RSM). Based on statistical analysis, optimal conditions were determined to be 42.5, 3.28 mM, 32.95 m℃g/mL and 164 rpm, respectively. Under optimal reaction conditions, the concentration of (S)-naproxen produced by the hydrolytic reaction of (R,S)-naproxen 2,2,2-trifluoroethyl thioester was 0.260 mM, similar to that of (S)-naproxen (0.266 mM) predicted by the model.