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HPLC Application of Newly Developed D-Phenylalanine Imprinted Polymeric Microbeads Prepared by a Novel Modified Suspension Polymerization

초록

영어

Molecular imprinting is a versatile and facile technique for the preparation of synthetic polymers with predetermined molecular recognition sites for a desired template. It enables the formation of tailor-made materials, which can be used as chiral stationary phases in various chromatographic techniques. In our previous investigations, MIP microbeads were prepared by a modified suspension polymerization method using D-Phe as template. In the current study, these microbeads, packed into a stainless steel column, were evaluated as HPLC stationary phase for the enantioseparation of phenylalanine isomers. The selection of a suitable mobile phase, mobile phase composition, pH and flow rate were investigated for the best resolution. Baseline separation was achieved with EtOH-acetate buffer solution as a mobile phase. Maximum resolution was obtained for a mobile phase of pH 4.75. The separation factor and resolution decreased with an increase in the mobile phase flow rate. These microbeads were found superior to the majority of the reported molecularly imprinted polymers with respect to chiral separation ability.

저자정보

  • Hamayun Khan Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan
  • Taous Khan Department of Chemical Engineering, Kyungpook National University
  • Jae-Seung Lee Department of Chemical Engineering, Kyungpook National University
  • Joong Kon Park Department of Chemical Engineering, Kyungpook National University

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