원문정보
초록
영어
A novel purification method was developed for producing (+)-dihydromyricetin, an active component in the fruit of Hovenia dulcis, giving high purity and yield. The developed method, which consisted of fractional precipitation and chromatography, was found to be a simple and efficient procedure for the isolation and purification of (+)-dihydromyricetin from the crude extracts. The use of fractional precipitation in the pre-purification process allowed for the
efficient separation of (+)-dihydromyricetin from interfering compounds and dramatically increased the purity of (+)-dihydromyricetin compared to alternative processes. This is a fractional precipitation of (+)-dihydromyricetin by difference of solubility in acetone solution. The crude extracts were efficiently pre-purified, increasing in purity from 55.7% to over 84.9% with a yield of 97% by fractional precipitation. Fractional precipitation was performed to obtain
(+)-dihydromyricetin with a high purity, which is adjustable to apply to the column employed in a subsequent HPLC purification step.