원문정보
초록
영어
Immuno-stimulating activities of thiolated chitosan microspheres loading Bordetella bronchiseptica were evaluated by measuring the mucosal immune response following nasal administration in mice. Thiolated chitosan microspheres were prepared according to the ionotropic gelation of low molecular chitosan with tripolyphosphate anions1). The thiol group was induced from 2-iminothiolane hydrochloride. The particle size distributions of thiolated chitosan microspheres were analyzed using an electrophotometic light scattering spectrophotometer, Photal, ELS-8000, Osaka). The contents of thiol group in chitosan microsphere were measured by Ellman’s assay method. The level of antigen-specific antivodies (IgA and IgG) in serum, saliva, and nasal wash samples was determined using enzyme-linked immunoabsorbent assay (ELISA).