원문정보
초록
영어
The osteoinductive activity of bone morphogenetic proteins (BMPs) has been tested both in vivo and in vitro. In the generally accepted in vivo experiments, BMPs are implanted with various types of carrier systems in muscles of a rodent species and new bone formation is analysed after a few weeks. in vivo assays, however, are not always indicative of in vivo osteoinduction, because in vivo various other components of the extracellualr matrix influence the commitment and differentiation of progenitor cells. But, cell cultures could provide a well-controlled and adjustable environment in which to study the activities of BMPs. One marker, which is usually defined by measuring the expression of one or more markers of metabolism in functionally mature cartilage or bone cells, is alkaline phophatase (ALP). The recent studies have been reported that at least ALP activity measurements in mouse myoblasts have produced an adequate correlation to the results of in vivo bone formation assays. The results demonstrate that native bovine BMPs were responsible for the increase in ALP activity in cell lines.