원문정보
초록
영어
Porcine epidemic diarrhae virus(PEDV) is an infectious, highly contagious virus of swine that causes acute diarrhea and dehydration, leading to death with high mortality. Cholera toxin B subunit (CTB) has been used extensively as a carrier for fused peptide immunogens in vaccine research. CTB binds to the GM1 ganglioside receptor present on the intestinal epithelial surface, leading the elicitation of mucosal immune response to pathogens and enhancing the immune
response. The plant binary vector, pMYV514, which contains synthetic CTB-neutralizing epitope of PEDV fusion gene (sCTB-COE) under the control of ubiquitin promoter, was constructed and transformed into lettuce (Lactuca sativa L.) by Agrobacterium-mediated transformation method. The stable integration and transcriptional expression of the sCTB-COE fusion gene were
confirmed by genomic DNA PCR and Northern blot analyses, respectively. The monomer and pentamer of CTB-COE fusion protein were detected in Western blot analysis. GM1-ganglioside ELISA confirmed that plant-synthesized pentameric CTB-COE fusion protein bound to the intestinal membrane GM1-ganglioside receptor and the highest expression level of pentameric
CTB-COE fusion protein was 0.1% of total soluble protein in the leaf tissues of lettuce. These results demonstrate that the transgenic lettuce expressed CTB-COE fusion protein and could be consumed as edible plant vaccines to induce mucosal immunity in the form of raw plant materials .