earticle

영어

The vaccine of Japanese Encephalitis Virus (JEV) is produced in the brain of mouse or fertilized eggs currently. However the conventional processes have many problems, such as the high cost of production, the controversy over bioethics for sacrificing animals and the impurities derived from mouse brain may cause neurological diseases. These problems could be resolved by the roduction system for JE vaccine derived from Vero cells with serum free media. In this work, the optimum condition of JE vaccine production is established and the assay titrating JEV for rapid detection would be developed.
The Vero cell growth kinetics and infectivity of JEV strain against the Vero cells were determined by measuring cell density and JEV infectious titers. The immunogenicity of Vero cell-derived JEV was determined by ELISA method with an E-antigen. The specific protein of JEV was detected by using western blotting.