원문정보
초록
영어
Calcium is a critical parameter which is required for influencing the interaction of both cell-cell and cell-matrix. Therefore extracellular calcium has strong potentiation effects on cell adhesion and cell aggregation for diverse cell types in vitro.1) Cell aggregation during cell culture causes
obstruction to nutrient and oxygen transfer, leading to cease of the cell growth and eventually cell death. It has been reported that low calcium levels in serum-free media reduces aggregation of HEK293 cells2) and chondrocytes.1) and in the preceding study, we observed the effect of
calcium on cell growth and aggregation of Chinese Hamster Ovary(CHO) cells. In preexisting commercial media which the concentration of calcium is high, cell aggregation occurred and cell growth was low. On the other hand, when the concentration of calcium was relatively low, the cell growth was higher and the cells average diameter maintained uniformly. In this study, a
same CHO-DG44 cell line, but selected by a different route was used in suspension culture to observe how the optimum concentration of calcium differed when the aggregation appearances are more severe. We also observed how the phenomenon of aggregation changed by addition of
substances like dextran sulfate which reduces cell aggregation.