원문정보
초록
영어
The major factor which drives the functional and pathological alterations of renal system in diabetic nephropathy(DN) is hyperglycemia. Most of studies are focusing on the glomerular abnormality of DN, yet the tubulointerstitial rather than glomerular pathology is more related to the decline of renal functions in DN. It suggests the involvement of the tubulointerstitium in DN with nephromegaly in the early stages of diabetes although the etiology of the tubulointerstitial pathology in DN is not fully understood. In this study, we constructed an initial 2-DE reference map for primitively cultured human proximal tubular cell (HK-2) to make DN pathways more completely understood. 2-DE is done in the presence of 5mM and 30mM glucose respectively
corresponding to blood glucose concentrations in the early and late stages of diabetes. HK-2 cellular proteins, which are expressed showing a distinct difference, were extracted and identified by ESI-Q-TOF MS/MS. The difference between the protein expressions of low and high glucose concentrations will provide a greater understanding of DN pathways in HK-2 cell at the condition of hyperglycemia.