원문정보
초록
영어
The production level of torulene, a monocyclic carotenoid, in recombinant Escherichia coli were investigated by modulating expression level of enzymes, culture conditions and engineering of the isoprenoid precursor pool. The Expression level of a mutant lycopene cyclase significantly changed the torulene profile. Torulene was produced better at 28oC than 37oC while initial culture pHs didn’t significantly affect on torulene production. Torulene and other carotenoids
(lycopene, tetradehydrolycopene and b-carotene) production was severely repressed in E. coli grown in LB, 2X YT, TB and MR media containing glucose. In contrast, glycerol-containing LB, 2X YT, TB and MR media enhanced torulene production up to 10%. Overexpression of dxs, dxr, idi and/or ispA, individually and combinatorially, enhanced torulene production up to 3.1-3.3 fold. The High torulene formation was observed in a high DO level bioreactor in TB and MR media
containing glycerol. Lycopene was efficiently converted into torulene during aerobic cultures, indicating that the engineered torulene pathway is well coordinated and the functionality and integrity of the carotenogenic enzyme complex are well maintained.