원문정보
초록
영어
Doxorubicin(DXR) and daunorubicin(DNR) are anthracycline antibiotics produced by Streptomyces peucetius and widely used as cancer chemotherapeutic agents. Although DXR/DNR regulators have been mainly studied, other factors, such as regulations in central metabolic pathway and carbohydrate metabolism, are known to be important for improving their productivity. To study the correlations between those pathways and DXR/DNR productivity, we selected 268 genes involved in glycolysis, fermentation, citric acid cycle, butanoate metabolism, and etc, and searched for their sequences from the S. peucetius genome by comparing gene sequences of S. coelicolor. Those genes were amplified by PCR and arrayed on the glass slides. We monitored gene expression changes of those genes in two different conditions, R2YE and NDYE media. The analyzed data suggested that the expression levels of genes in alanine and glutamate pathways might be important in DXR/DNR productivity.