earticle

영어

Water-soluble intracellular polysaccharides(IPS) obtained from the mycelia of Inonotus obliquus belonging to basidomtces fungus was proved to be clinically important agent due to its antidiabetic activity. During the strain improvement program, it was found that cell mass increase was most important in order to enhance IPS productivity, since IPS amount was almost constant per unit cell mass. Therefore, attempts to enhance biomass amount were carried out in the
shake flask cultures of Inonotus obliquus mycelium. For the optimization of IPS production medium, sequential statistical methods were adopted, such as one-factor-at-a-time method(OFAT), Plackett-Burman design, full or fractional factorial design(FFD), steepest ascent method(SAM) and Response surface method(RSM). About 2-fold increase in the IPS production was observed in the final optimized medium, as compared to the IPS amount obtained by use of the
previous medium. Notably, It was observed that the producers should be proliferated in compact-pelleted morphology of the optimum size (0.5~1mm in diameter) for the maximum production during the whole period of fermentation.