원문정보
초록
영어
1,2-Propanediol (1,2-PD, propylene glycol) is a chemical which has three carbon-diol and it contains a stereo structure at center of carbon. It is mostly used in unsaturated polyester resins, cosmetics, liquid detergents, detergents and solvent for unstable to water in pharmaceutical industry. Saccharomyces cerevisiae is a well known and a useful strain for industrial fermentation. Since S. cerevisae does not have genes necessary for the production of
1,2-propanediol, it is essential to import the genes from 1,2-propanediol producing cells. The objective of this research is to construct a plasmid for S. cerevisae to produce 1,2-propanediol by genetic manipulation. For this work, the key enzymes in the metabolic pathway of S. cerevisiae were selected for the production of 1,2-propanediol methylglyoxal synthase and glycerol dehydrogenase. Methylglyoxal synthase convert dihydroxyacetonephosphate
(DHAP) to methylglyoxal. Glycerol dehydrogenase is involved in the conversion of glycerol to dihydroxyacetone. The genes which encode methylglyoxal synthase (mgs) and glycerol dehydrogenase (gldA) were obtained from Escherichia coli. The genes were cloned in a pESC-URA vector and transformed to S. cerevisiae.