원문정보
초록
영어
1,2-Propanediol has been used in unsaturated polyester resins, liquid laundry detergents, cosmetics, and pharmaceutical manufacturing as a solvent for drugs unstable in water. Several organisms are reported to ferment common sugars to 1,2-PD. However, the titers are fairy low and the organism is not characterized well enough to enable the improvement of the 1,2-PD production by metabolic engineering1). S. cerevisiae has a well established genetics system and has been completely sequenced2). In this work, a strain of Saccharomyces cerevisiae was
engineered for the production of 1,2-PD. For the production of 1,2-PD, methylglyoxal synthase (mgs) and glycerol dehydrogenase (gldA) from Escherichia coli were cloned onto high copy number vector pYES2/CT and pYES3/CT plasmid respectively. After introducing the mgs and gldA genes, production of 1,2-PD was analyzed by HPLC.