원문정보
초록
영어
Poly-γ-glutamic acid (γ-PGA) is an unusual anionic polypeptide in which glutamate is polymerized via γ-amide linkages and it is synthesized by the γ-PGA synthetase complex (PgsBCA). Coryneform bacteria is the best host for the surface display of pgsBCA γ-PGA synthetase motif and has several advantages over other bacteria. It is generally recognized as safe. And Coryneform bacteria has the ability to produce high levels of glutamic acid, so it can be used to synthesize γ-PGA directly. So we constructed the recombinant Corynebacterium glutamicum expressed the pgsBCA complex, encoding the PGA synthesis enzymes. Second, its fermentation characteristics are well-known and established technically. The recombinant of C. glutamicum extracellularly produced γ-PGA in glutamate-independent γ-PGA production media. The recombinant of C. glutamicum produced 2.5 g/l of γ-PGA in the glutamate-independent γ-PGA production media, and the molecular weight of γ-PGA was determined over 8,300 kDa by GPC. And interestingly, the produced γ-PGA was the L-type γ-PGA, more than 90 % of L-glu content. These results are suggested the L-type γ-PGA is useful material for developing various applications and studies of structure. [This work was supported by the 'Seoul R&BD Program(10580)’]