원문정보
초록
영어
the purpose of this study was to assess the effects of a herb remedy . on the cell proliferation, inducible nitric oxide synthase (inos) mRNA gene expression and nitric oxide (NO) production in RAW 264.7 macrophage cells. For the screening of anti-inflammation, a ethanolic extract of traditional herbal medicine was examined its inhibitory effect, and we confirmed that 1 herb.
NO concentrations were determined by measuring the amount of nitrite in the LPS treated-macrophage cell culture supernatant using Griess reagent. In cytotoxicity test, with the a herbal remedy concentration of up to 500 mg/mL, no significant effect on RAW 264.7 macrophage cells morphology and viability was observed compared to the control during the culture period.
Also, more than the concentration of 125ppm, a herbal remedy was significantly decreased the production of NO. As a result, we knew inhibitory effects of a herbal remedy extracts on Nitric Oxide synthesis in RAW 264.7 macrophage cells.