원문정보
초록
영어
In previous studies, we mutagenized Bradyrhizobium japonicum 61A101C with the transposon Tn5 and isolated JS314, the LPS- mutant strain, which lacks the O-polysaccharide chain. Subsequently, the interrupted gene was cloned and identified as rfaF gene, and used to restore the phenotype of the wild-type strain. Since it has been reported that LPS- mutants of Ralstonia solanacearum were more sensitive to antimicrobial plant peptides, in this study, we compared
the susceptibility of the three B. japonicum strains - 61A101C(wild-type), JS314(LPS- mutant) and JS314/pLps(complemented strain) - to purothionin, one of antimicrobial peptides. In the presence of 10μM and 20μM purothionin, JS314 was much more sensitive to the peptide than other strains: the relative growth (%) of JS314 was zero, but those of others were above 50%. We showed that an LPS- mutant of B. japonicum was sensitive to an antimicrobial peptide as
that of R. solanacearum had been. Therefore, the disruption of the rfaF gene involved in LPS biosynthesis appears to render bradyrhizobia more sensitive to antimicrobial peptides.