earticle

영어

Clostridium difficile (C.difficile) is not only the most common infectious cause of nosocomial diarrhea, accounting for 20~25% of all antibiotic associated diarrhea, but also identified in 95% of pseudomembranous colitis(PMC) cases. In particular, there has been a recent increase in the number of children and infants suffering from C.difficile-related infection during antibiotics treatment. The overuse of antibiotics may alter the normal intestinal flora and increase the risk of developing C.difficile diarrhea. Although antibiotics (vancomycin, etc.) have various problem such as high medical costs, antibiotic resistance, and relapses or re-infections during treatment periods, new alternative agents for treatment of C.difficile associated diarrhea or infection
diseases was not yet determined. Therefore, in this study, we confirm the inhibitory activity through the AG ethanol extract. In order to verify the effect, we will use test methods such as paper disc diffusion method, MIC(Minimum Inhibitory concentration) method and liquid medium
dillution method. The ethanol soluble fractionate that is obtained by the traditional herbal medicines have high inhibitory activity for C.difficile about 93.9±1.7. Also, AG have the high inhibitory activity against growth of S. aureus. S. aureus with Salmonella spp., vibrio parahaemolyticus is food poisoning bacillus. We will test the antimicrobial effect about the AG of C.difficile and food poisoning bacillus. Also, we will test lactobacillus that is affected by extract which shows high inhibitory activity for the C.difficile.