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논문검색

Genetic Characterization and Spore Production Optimization of Antifungal Polyene-producing Actinomycetes

초록

영어

The polyene antifungal antibiotics, mostly produced by Gram-positive soil actinomycetes, are a family of type I polyketide macrolide ring compounds with 20-40 carbon backbone contain 3-8 conjugated double bonds. Using polyene-specific genomic screening strategy, we previously isolated three novel polyene-producing actinomycetes strains from soil, implying the potential
application of these strains’ spores as microbial pesticides. Among three isolated strains, an approximately 112.9 kb contiguous nystatin-like CPP (Cryptic Pseudonocardia Polyene) biosynthetic gene cluster in P. autotrohpica and a 44.8kb FR008-like polyene biosynthetic gene cluster in Streptomyces MMBL003 have been isolated and genetically characterized. Using a fractional factorial design, both KH2PO4 and yeast extract were identified to be the key factors
influencing CPP productivity in P. autotrophica. In addition, we confirmed that the sonication was a very efficient method for actinomycetes spore generation with a sonicator power-dependent manner. These sonication-driven actinomycetes spores retained significant portion of their cell viabilities as well as antifungal activities after freeze-drying procedure, implying the potential
application of these strains’ spores as microbial pesticides.

저자정보

  • Byung-Kyun Kim Department of Biotechnology, Inha University
  • Ja-Ryong Koo Department of Biotechnology, Inha University
  • Kyuboem Han Hanson Biotech Co., Ltd.
  • Eung-Soo Kim Department of Biotechnology, Inha University

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