earticle

영어

Fish Cytochrome P450 1A (CYP1A) genes have been widely used to identify the effect of polycyclic aromatic hydrocarbons, including suspected endocrine-disrupting chemicals (EDCs)1. CYP1A are haem-binding proteins, involving in detoxification of organic compounds such as dioxin, PAH-like compounds. In this study, full-length cDNA of CYP1A gene was cloned from bagrid catfish, Pseudobagrus fulvidraco and its gene expression was measured using real time RT-PCR after exposure of beta-naphthoflavone (BNF) (50mg/kg) for 96h. P. fulvidraco CYP1A gene consists of 1578 bp of open reading frame (ORF), encoding 525 aa of putative protein, 124bp of 5’-UTR and 1058bp of long 3’-UTR. On the phylogenetic tree, P. fulvidraco CYP1A gene was clustered with goldfish and zebrafish with 76% of sequence identity using ClustalX. P. fulvidraco CYP1A transcripts was ubiquitously distributed in seven tissues (brain, gill, intestine, liver, kidney, skin and muscle), but especially high in liver. In the response to BNF exposure, induction of P. fulvidraco CYP1A mRNA expression was detected in BNF-exposed brain, kidney and liver in time-dependent manner. Kidney-CYP1A mRNA expression was steeply increased within 6h after exposure and then gradually decreased. On the other hand, brain- and liver-CYP1A mRNA expression was time-dependently increased until 24h after exposure, especially showing sharply increase in liver. These findings indicate that P. fulvidraco CYP1A gene expression would be a useful biomarker for assessing BNF toxicity.