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영어

Shigella sonnei is a gram-negative facultatively intracellular pathogen responsible for bacillary dysentery in humans. S. sonnei is a significant cause of gastroenteritis in both developing and industrialized countries. High priority has been given by the world health organization to the development of vaccines to control Shigellosis caused by these bacteria. Knowledge of the diversity and antimicrobial susceptibility of the bacterium may be helpful in the management of both individual cases and outbreaks. However, information regarding to profile of immunogenic proteins of Shigella is not available now. In this study, immunoproteomic approach using Two-dimensional electrophoresis (2-DE) followed by immunoblotting, was employed to investigate the pathogenicity and immunogenicity of intracellular proteins (ICP), outer membrane proteins (OMP) and extracellular proteins (ECP) in S. sonnei. ICP, OMP, ECP extractions of S. sonnei were separated using 2-DE immunoblot assay. Our results indicated that 42 immunogens were detected in which 19 proteins, 12 proteins, 11 proteins from ICP, OMP, and ECP, respectively. The MALDI-TOF-MS analysis for these protein spot using ExPASy database enabled proteins identification. We suggest that the proteins could be potential candidates for vaccine study. Moreover, our immunoproteomic approach study may be suggesting a good method to find the vaccine candidate proteins from the other pathogen.