원문정보
초록
영어
In the previous study, we have cloned a recombinant E. coli harboring flavin-containing monooxygenase (FMO) gene. Recombinant E. coli produced 920 mg/L of Bio-indigo and 5 mg/L indirubin for 20 h cultivation on the tryptophan medium (0.2% tryptophan, 1% NaCl, 0.5% yeast extract). To enhance indirubin production, we observed the effect of amino acids on the tryptophan medium composition. Cysteine, asparatic acid and arginine significantly enhanced the
indirubin production. Concentrations of amino acids were optimized by response surface methodology. The optimal amino acid combinations on the tryptophan medium were determined as cysteine 0.1% (w/v), aspratic acid 0.05% (w/v) and arginine 0.05% (w/v), respectively. Under this condition, 96 mg/L indirubin and 6mg/L indigo were produced.