원문정보
초록
영어
The bacterial ghost system is a novel vaccine delivery system. It provide versatile carrier functions for foreign antigens with excellent natural intrinsic adjuvant properties. The optimal batch process for high cell density cultivation of E. coli DH5 /pHCE-InaN-GAPDH-Ghost37 was developed. The α optimal parameters for the ghost fermentation were determined as 1 g/l glucose in LB broth, 300 rpm agitation and 1 vvm aeration using 5L-jar fermenter. And the maximum ghost vaccine cell mass was obtained about 2.18 g/l. But the productivities of ghost
vaccine cell mass were not enough to industrialization.
Therefore, construction of new ghost bacteria with another E. coli XL1-Blue strain for high cell density culture was change and the feeding strategy with Riesenberg's defined medium during fed-batch process was evaluated. The fermentation were conducted in three phases. The initial phase (phaseⅠ) was the batch growth phase. Phase Ⅱ was a fed-batch phase with an exponential feeding rate to control the specific cell growth rate to 0.15h-1. Phase Ⅲ was the induction phase. In this phase, the specific growth rate was reduced to 0.03h-1 and the
temperature was shifted up to 42℃.
The components of the optimized semi-defined medium for E. coli XL1-Blue /pHCE-InaN-GAPDH-Ghost37 were determined as 20 g/l glucose, 10 g/l yeast extract, 250mg/l thiamine with Riesenberg's defined medium. The optimal batch fermentation parameters were determined as 10% inoculum size, 300 rpm agitation and 1.5 vvm aeration, respectively. After the batch process was optimized using modified Riesenber' defined medium, the maximum ghost vaccine cell mass was obtained about 5.5 g/l. And it shows that the bacterial ghosts with E. coli can be
used as effective and commercially available vaccine in aquaculture.