원문정보
초록
영어
An efficient succinic acid producer, Mannheimia succiniciproducens MBEL55E, was isolated from bovine rumen of Korean cow. Recently, a genetically engineered succinic acid overproducing mutant, M. succiniciproducens LPK7, was also developed based on full genome sequence [1]. In this study, 2-DE and LC-MS/MS were used to analyze proteome of the mutant cells at the exponential and stationary phases. These results were compared with those of
the wild type strain (MBEL55E) to elucidate the global physiological and metabolic changes responsible for succinic acid overproduction. Several features, which correspond to the trend of metabolite production and growth profiles, and indispensable characteristics for further strain improvements will be described in detail. [This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (2005-01294). Further supports by the LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.]