원문정보
초록
영어
Chemical synthesis of 1,2-propanediol (1,2-PD, propylene glycol) that is most commodity chemicals have some problems [1]. In this work, microbial fermentation was used form the production of useful industrial chemicals [2]. Saccharomyces cerevisiae was selected a host strain. Methylglyoxal synthase (mgs) and glycerol dehydrogenase (gldA), the two enzymes were selected for the production of 1,2-PD metabolism. And the two enzymes that are essential
intermediate from DHAP and glycerol dehydrogenase [2-4]. The yeast expression vector, pESC-URA has multi-cloning sites, was used construction plasmid containing target gene mgs and gldA. The plasmid containing mgs and gldA was inserted into S. cerevisiae by LiAc/SS-carrier DNA/PEG transformation. And then strain containing plasmid was screened by SDC (A,T)
medium. The culture medium was YPD adding galactose as trigger for the activation of GAL promoter in the plasmid [5]. The concentration of 1,2-PD was analyzed by HPLC with refractive index detector. The mobile phase was 0.5M sulfuric acid and flow rate was 0.5 ml/min. After growing the yeast, we broke the cells to extract 1, 2-PD. The solution for 1,2-PD analysis was condensed and analyzed by HPLC. Cells containing mgs or gldA or two genes in pESC-URA vector were shown the production of 1,2-PD.