원문정보
초록
영어
Production of biohydrogen from formate by catalysis of formate hydrogenlyase is very attractive reaction in application of compact and mobile fuel cell system. Because this reaction is very fast and recovery of H2 is easy since the CO2 is the only by-product. In addition, formate as law
material is less bulky than H2. In this study, we examined the potential of H2 production from formate for application of fuel cell using immobilized cell.
We used the newly developed strain E.coli SH5 which is deleted two uptake hydrogenase, ldhA, frdAB, and hycA gene. For the higher stability and volumetric production rate, we entrapped SH5 in alginate and measured H2 productivity. Specific H2 production activity of SH5 in free cell and
immobilized cell condition were 1.2 and 0.2 μmol/mg/min, respectively. When immobilized cell used, increase of formate concentration up to 200 mM did not reduced cell activity and maximum volumetric production rate (25 ml/h per 25 ml reaction volume). It is higher than previous reported result (10ml/h per 20ml reaction volume)1 by 2 fold. It means that this system is very potential to apply for compact and mobile energy generation.