원문정보
초록
영어
Enhancement of hydrogen production yield from glucose was studied for a series of Escherichia coli cells obtained by disruption of uptake hydrogenases and redistribution of carbon metabolic fluxes. Culture experiments were conducted in a batch mode with 10 g/L glucose and 3 g/L yeast extract. When hydrogenase 1 (hya) and hydrogenase 2 (hyb) which consume hydrogen for cellular energy recycling were inactivated, the hydrogen production yield increased to 1.48
mol/mol glucose from 1.20 mol/mol glucose, the yield of wild-type E. coli strain.
When lactate dehydrogenase (ldh) and fumarate reductase (frd) were disrupted from the E. coli △hya△hyb, the yield further increased to 1.80 mol/mol glucose. The final construct E. coli △hya△hyb△ldh△frd was cultivated under reduced hydrogen partial pressure and it was observed that the hydrogen yield was enhanced to 2.11 mol/mol glucose. This is higher than the theoretical maximum of E. coli which is 2.00 mol/mol glucose. The analyses of carbon metabolites revealed that, in the final E. coli construct, most carbons were directed to the
metabolic pathways leading to hydrogen production by formate hydrogen lyase.