원문정보
초록
영어
Cancer that is one of serious diseases for human causes angiogenesis, metastasis and abnormal cell divisions including genetic change. Thus lots of researchers have been investigated to inhibit cancer. Human 90K (h90K) protein has very crucial role for inhibition of cancer metastasis. h90K is a glycoprotein having 7 N-glycosylation sites. In this work, we performed expression of recombinant h90K glycoprotein in Drosophila S2 cell system. We constructed stably-transfected S2-h90K cells by antibiotic selection and confirmed h90K expression using Western Blot analyses with hexa Histidine tag and bioactivity check. We also identified glycosylation patterns of recombinant h90K glycoprotein using HPLC and MALDI-MS.