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Expression & Analysis of Recombinant Human 90K Glycoprotein In Drosophila S2 Cells

초록

영어

Cancer that is one of serious diseases for human causes angiogenesis, metastasis and abnormal cell divisions including genetic change. Thus lots of researchers have been investigated to inhibit cancer. Human 90K (h90K) protein has very crucial role for inhibition of cancer metastasis. h90K is a glycoprotein having 7 N-glycosylation sites. In this work, we performed expression of recombinant h90K glycoprotein in Drosophila S2 cell system. We constructed stably-transfected S2-h90K cells by antibiotic selection and confirmed h90K expression using Western Blot analyses with hexa Histidine tag and bioactivity check. We also identified glycosylation patterns of recombinant h90K glycoprotein using HPLC and MALDI-MS.

저자정보

  • Kyoung Ro Kim Dept. of Chemical Engineering, Pohang University of Science and Technology (POSTECH)
  • Yeon Kyu Kim Dept. of Chemical Engineering, Pohang University of Science and Technology (POSTECH)
  • Dong Gyun Kang Dept. of Chemical Engineering, Pohang University of Science and Technology (POSTECH)
  • So Young Jang Proteomics team, Korea Basic Science Institute (KBSI)
  • Young Hwan Kim Proteomics team, Korea Basic Science Institute (KBSI)
  • Hyung Joon Cha Dept. of Chemical Engineering, Pohang University of Science and Technology (POSTECH)

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