원문정보
초록
영어
Several assays have been developed to measure the antioxidative activity of phenolic compounds. These assays focus on different mechanism of antioxidative reaction, for
example, scavenging of oxygen and hydroxyl radicals, reduction, chelation of metal ions,
and enzyme activity. This study evaluated the relationship between antioxidative activity
of a phenolic compounds and their chemical structures using ABTS ((2,2'-azinobis
(3-ethylbenzothiazoline-6-sulfonic acid), DMPD (n,n-Dimethyl-p-phenylenediamine), DPPH
(2,2-diphenyl-1-picryl hydrazyl) and FRAP (Ferric reducing antioxidant power) assay.
The antioxidants of commercial flavonoids (quercetin, kaempferol, (-)-epicatechin,
(+)-catechin) and phenolic acids (gallic acid, p-coumaric acid) were used to evaluate the
activity. The results suggest that the more hydroxyl substitutions, the stronger the
antioxidative activities by all assays. Also, the di-OH substitution at 4',5'-groups on the
B-ring by ABTS and DPPH assay and 3',4'-groups on the B-ring by FRAP and DMPD
assay are the most important for the antioxidative activity. This study confirmed that the
differences in antioxidative activity of the tested phenolic compounds could be attributed
to structural differences in number and position of hydroxyl substitutions.