원문정보
초록
영어
Most hematopoietic CD34+ cells are located in bone marrow (BM) architecture in close proximity to the endosteal surface of bone.1) On the other hand, periosteum contains two layers, an inner cambium layer and an outer fibrous layer stick to the outer surface of bone. Recently, we have
isolated and characterized periosteum-derived progenitor cells (PDPCs), that could be directly differentiated into chondrocyte and osteoblast.2) Furthermore, It was reported that osteoblastic cells regulate the hematopoietic stem cells niche.1) For these reasons, we investigated the synergistic effects of mesenchymal stem cells (MSCs) from BM and PDPCs as mixed feeder cells on ex vivo expansion of CD34+ cells from umbilical cord blood (UCB).
UCB-derived CD34+ enriched cells were cultured in a serum-free medium for 2 weeks over the respective mitomycin C-treated each or mixed feeder layers. We analyzed every 3 days for total cell expansion, immunophenotypes, and clonogenic ability. Ex vivo expansion of CD34+ cells was significantly enhanced with mixed feeder cells than with BM-derive MSCs alone. These
results indicate that mixed cells from near the both side of bone may be used as an efficient feeder cells for the expansion of CD34+ cells in vitro.