원문정보
초록
영어
Enzymes are recognized as useful tools for accomplishing industrially important chemical reactions. One of the major challenges in biocatalytic processes is to improve the activity and stability of enzymes. In this work, we have developed two systems for enzyme aggregate coating on the surfaces of electrospun nanofibers and magnetic beads. Candida rugosa lipase immobilized onto PS+PSMA nanofibers and magnetic beads. This method employs covalent
attachment of lipase onto nanofibers consisting of a mixture of polystyrene and poly(styrene-co-maleic anhydride). Lipase was immobilized on the surface of electrospun polymer nanofibers by using cross-linking enzyme aggregation by glutaraldehyde and they are covalently attached to surfaces of nanofibers. In addition, we immobilized lipase onto magnetic beads by glutaraldehyde.
Michaelis-Menten constant (Km) value of immobilized lipase was found to be greater than free lipase. According to comparison of stability of immobilized lipase onto nanofiber and immobilized lipase onto magnetic beads at room temperature, both immobilized lipase are more stable than that of the free lipase.
In case of immobilized lipase onto magnetic bead, it was stored at room temperature, the residual activity after 30 days was 55%, after 30th reuse of immobilized lipase, and the retained activity was 62%. and immobilized lipase onto PS+PSMA nanofibers was more improved stability and reusability, the residual activity after 30 days was 70%, after 30th reuse of immobilized lipase,
the retained activity was 68%. As the result, we significantly observed that immobilized lipase shows high activity recovery and good stability during the repeated use.