원문정보
초록
영어
Various chemocatalytic and biocatalytic methods have been developed for preparing chiral epoxides. Among biocatatytic methods, the kinetic resolution of racemic epoxides via an enantioselective hydrolysis reaction by an epoxide hydrolase(EH) is a very promising method since enantiopure epoxides with a high optical purity can be obtained from cheap and readily available racemic epoxides. A recombinant Escherichia coli possessing enantioselective epoxide hydrolase gene of Rhodotorula glutinis was used for producing chiral styrene oxide by asymmetric resolution of racemic styrene oxide.
The culture of recombinant E. coli in low temperature induced the expression of epoxide hydrolase protein with soluble form more than that in high temperature. The hydrolytic activity and SDS-PAGE showed that the culture temperature is related to the expression of soluble protein. Acknowldegement: This work was supported by the Marine and Extreme Genome Reserch Center Program, Ministry of Marine Affairs and Fisher. Republic of Korea.