earticle

영어

Hydrogen energy is the most efficient substitute energy as an alternative plan about environmental pollution and energy problem that is facing current. This study achieved hydrogen production research through mixing cultivation of Enterobacter aerogenes and Clostridium butyricum as a representative anaerobe microorganism. Clostridium butyricum that is strict anaerobe microorganism is usually more efficient than Enterobacter aerogenes that is facultative anaerobe microorganism in microbial singleness cultivation of hydrogen production. A reason of mixture cultivates two microbes that leads hydrogen production efficiency in secondary metabolism process from growth process of two microbes. The hydrogen yield which leads a mixture cultivation is visible the efficiency which is higher than singleness cultivation but it is a little different.
Hereupon through the Gram staining, it examines the growth pattern and selection of dominant species which plan a high hydrogen production yield. According to selected the Clostridium butyricum is the inferiority species microbe, it confirmed the hydrogen production yield that comes to be low from mixture cultivation. It means that will be able to attain the high hydrogen
production yield by phased cultivation. From culture medium, first Clostridium butyricum, a inferiority species cultivates. After Clostridium butyricum grew, Enterobacter aerogenes, a dominant species, cultivates. This research get higher hydrogen production yield. The result of hydrogen production yield is 1.4 mole H2 / mole glucose in case of the same time cultivation and 1.5 mole H2 / mole glucose in case of phased cultivation.