earticle

영어

The physical limitation of conventional lithographic top-down approaches for molecular scale tasks has spurred the development of alternative bottom-up strategies by exploiting molecular recognition and self-assembly of molecular building blocks found in biological systems.1-2 The programmability of molecular assembly pattern of DNA3, which is based on specific complementarity between bases, makes it a promising material for nanoscale manipulation and fabrication in which DNA serves as building blocks for nanostructures.4,5 Here, we developed three-dimensional protein networks that are amenable to global structural changes by the presence of target biochemicals. Streptavidin, as a model protein, is crosslinked by biotinylated DNA oligonucleotides, leading to nanosized protein networks. One of the DNA crosslinkers carries an aptamer sequence against adenosine, so the protein networks can be disassembled by adenosine. Using both experimental and computational methods we optimized the DNA sequence design to achieve facile target-triggered disassembly of the protein networks. The resulting protein network switchable by adenosine showed complete target-induced disassembly of the whole network with a high target specificity, i.e. no other nucleoside works. The switchable protein network is promising as smart biomaterials for stimuli-responsive drug
delivery.