원문정보
초록
영어
Polyethylenimine (PEI) has been used non-viral gene delivery carrier. Although it showed high transfection efficiency in vitro, PEI makes damage to normal cell and tissues because of its strong interaction with cell membrane(1). In order to improve the transfection efficiency and to reduce the cytotoxicity of PEI, the PEI and all-trans-retinoic acid (ATRA) conjugates were synthesized by carbodiimido method. ATRA is transported by the retinoid-binding protein
(CRABP-Ⅱ)(2). CRABP-Ⅱ migrate to nucleus from cytoplasm. The synthesis was confirmed by NMR, FT-IR and UV-spectrophotometer. Thier physicochemical properties were characterized by gel electrophoresis, particle size and zeta potential to estimate a possibility as a gene carrier. The transfection efficiency of PRA in NIH3T3 cell was up to 1.5 times higher than that of PEI (N/P 15). The cytotoxicity of PRA was also measured by MTT assay. PRA conjugates showed lower cytotoxicity compared with the PEI. Therefore, the PRA conjugate may be good candidate for
non-viral gene carrier.