earticle

영어

Type 1 diabetes (T1D) is an autoimmune disease that results in selective destruction of insulin-producing pancreatic β-cells of Langerhans. Insulin deficiency and its action during progression of diabetes mellitus are accompanied with various metabolic abnormalities such as hyperglycaemia, hypertension and hyperlipidemia, consequently resulting in chronic complications. In this study, a streptozotocin (STZ)-induced diabetic rat model, having relatively lower diversity of individual than human, was used to screen key biomarkers of diabetic
complications. To compare the protein profiles of a healthy control group with those of 2, 4, 8 and 12 weeks STZ-treated rat groups, we conducted proteomic analysis of rat plasma using a two-dimensional gel electrophoresis (2DE). In diabetic groups, several spots were differentially expressed, which showed either 50% down-regulation or 100% up-regulation compared to those of the healthy group. The down-regulated proteins were identified via ESI-Q-TOF mass
spectrometry, which were reconfirmed by Western blotting. Furthermore, proteins identified as progressive biomarkers of diabetic complications were compared with those from human diabetic serum.